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Updated: Feb 6, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
In-capillary microextraction for direct mass spectrometry analysis of biological samples
Yue Ren1, Wenpeng Zhang1, Ziqing Lin2
1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China; Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Abstract:
Slug flow microextraction (SFME)-nanoESI was originally explored as a single-step sampling ionization method for MS analysis of biofluids. In this work, a comprehensive study and development of the SFME has been carried out. Revers-phase SFME was developed to analyze chemical compounds in oil samples. A three-phase SFME system was introduced as a suitable approach for analyzing polar compounds in biofluids. The impacts by the capillary inner diameter, solvent and sample properties were also investigated, leading the use of fused silica capillaries for performing SFME.
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