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Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
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A novel aqueous biphasic system formed by deep eutectic solvent and ionic liquid for DNA partitioning
Panli Xu1, Yuzhi Wang1, Jing Chen1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta
|August 9, 2018
Summary
Novel aqueous biphasic systems (ABSs) using ionic liquids (ILs) and deep eutectic solvents (DESs) efficiently extract DNA. These IL/DES systems achieve high DNA extraction efficiency and selective separation of nucleic acids from proteins.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Materials Science
Background:
- Aqueous biphasic systems (ABSs) are effective for biomolecule separation.
- Ionic liquids (ILs) and deep eutectic solvents (DESs) offer tunable properties for novel separation media.
Purpose of the Study:
- To develop and evaluate novel IL/DES-based ABSs for efficient DNA extraction.
- To investigate the factors influencing phase formation and DNA partitioning in these systems.
- To demonstrate the application of these systems for selective separation of nucleic acids and proteins and DNA extraction from biological samples.
Main Methods:
- Development of ILs/DESs based on betaine/carboxylic acid, betaine/carbohydrates, and [TBAB][PPG400].
- Evaluation of phase-formation ability of various ABSs.
- Optimization of DNA extraction conditions using factor experiments.
- Separation of mixed samples (DNA/cytochrome C, DNA/bovine hemoglobin).
- Application to DNA extraction from bovine whole blood.
- Mechanism exploration using FT-IR, CD, DLS, and TEM.
Main Results:
- The phase-forming ability of ABSs depends on IL anion alkyl chain size, DES viscosity, density, hydrophilicity, and inorganic salt properties.
- Optimized IL/DES-based ABS achieved a maximum DNA extraction efficiency of 99.60%.
- Selective separation of DNA from proteins was achieved, with DNA partitioning to the IL-rich phase, influenced by analyte isoelectric point and system pH.
- Successful application in extracting DNA from bovine whole blood was demonstrated.
Conclusions:
- Novel IL/DES-based ABSs provide an efficient and selective method for DNA extraction and purification.
- The developed systems offer a promising single-step approach for separating nucleic acids from proteins.
- These systems have broad potential applications in molecular biology, diagnostics, and other separation fields.
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