Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective antimitochondrial agents inhibit calmodulin.

W L Bodden, S T Palayoor, W N Hait

    Biochemical and Biophysical Research Communications
    |March 13, 1986
    PubMed
    Summary

    Rhodamine-123 and dequalinium, compounds that accumulate in tumor mitochondria, act as calmodulin antagonists. They inhibit energy production and cell proliferation, offering potential therapeutic strategies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Silencing of stathmin induces tumor-suppressor function in breast cancer cell lines harboring mutant p53.

    Oncogene·2006
    Same author

    People of the year. William N. Hait, MD, PhD. Interview by Bill Berlin.

    New Jersey medicine : the journal of the Medical Society of New Jersey·2005
    Same author

    Tubulin targeting agents.

    Cancer chemotherapy and biological response modifiers·2001
    Same author

    Activation of phospholipase C induces the expression of the multidrug resistance (MDR1) gene through the Raf-MAPK pathway.

    Molecular pharmacology·2001
    Same author

    The prognostic and predictive values of ECD-HER-2.

    Clinical cancer research : an official journal of the American Association for Cancer Research·2001
    Same author

    Interactions of 1-methyl-4-phenylpyridinium and other compounds with P-glycoprotein: relevance to toxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

    Brain research·2001

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pharmacology

    Background:

    • Cationic-lipophilic compounds accumulate in tumor mitochondria, inhibiting energy production.
    • Structural similarities exist between these compounds and known calmodulin inhibitors.

    Purpose of the Study:

    • To investigate if rhodamine-123 and dequalinium antagonize calmodulin activity.
    • To evaluate the antiproliferative effects of these compounds on C6 astrocytoma cells.

    Main Methods:

    • Assessed inhibition of calmodulin-stimulated cyclic nucleotide phosphodiesterase.
    • Performed kinetic analysis to determine the mechanism of inhibition.
    • Studied antiproliferative effects on the C6 astrocytoma cell line.

    Main Results:

    • Rhodamine-123 (IC50 = 58 microM) and dequalinium (IC50 = 1 microM) inhibited phosphodiesterase activity.
    • Dequalinium competitively inhibited calmodulin's activation of phosphodiesterase.
    • Both rhodamine-123 and dequalinium inhibited C6 astrocytoma cell proliferation, while propylinium did not.

    Conclusions:

    • Rhodamine-123 and dequalinium function as calmodulin antagonists.
    • These compounds inhibit cellular proliferation, suggesting potential anticancer applications.

    Related Experiment Videos