Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reduced rhodopsin phosphorylation during retinal dystrophy.

D J Takemoto, J Cunnick, L J Takemoto

    Biochemical and Biophysical Research Communications
    |March 28, 1986
    PubMed
    Summary

    Inherited retinal dystrophy in dogs involves decreased rhodopsin phosphorylation due to increased phosphatase activity, leading to vision loss. This biochemical change contributes to retinal degeneration.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Expression of the cholera toxin B subunit (CT-B) in maize seeds and a combined mucosal treatment against cholera and traveler's diarrhea.

    Plant cell reports·2011
    Same author

    Role of albumin as a fatty acid carrier for biosynthesis of lens lipids.

    Experimental eye research·2005
    Same author

    Glutathione peroxidase-1 deficiency leads to increased nuclear light scattering, membrane damage, and cataract formation in gene-knockout mice.

    Investigative ophthalmology & visual science·2001
    Same author

    PKC-gamma phosphorylation of connexin 46 in the lens cortex.

    Molecular vision·2001
    Same author

    PKCalpha and PKCgamma overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line.

    Molecular vision·2001
    Same author

    Protein kinase C alpha and gamma in N/N 1003A rabbit lens epithelial cell differentiation.

    Molecular vision·2001

    Area of Science:

    • Ophthalmology
    • Canine Genetics
    • Biochemistry

    Background:

    • Inherited retinal dystrophy in Irish Setters is linked to reduced cGMP phosphodiesterase (PDE) activity.
    • This leads to elevated cGMP levels and subsequent retinal degeneration.

    Purpose of the Study:

    • To investigate the biochemical basis of reduced rhodopsin phosphorylation in canine inherited retinal dystrophy.
    • To identify the specific molecular defect causing altered rhodopsin phosphorylation.

    Main Methods:

    • Retinas from affected and normal Irish Setter dogs were analyzed.
    • Phosphorylation levels of rhodopsin were assessed using gamma-32P-ATP and SDS-PAGE.
    • Experiments involved mixing affected and normal retinas to study inhibitory effects.

    Main Results:

    • Rhodopsin phosphorylation was significantly reduced in retinas from affected dogs.
    • Affected retinas inhibited rhodopsin phosphorylation in normal retinas when mixed.
    • This inhibitory effect was blocked by sodium fluoride (NaF), suggesting increased phosphatase activity.

    Conclusions:

    • Decreased rhodopsin phosphorylation in inherited retinal dystrophy is caused by elevated phosphatase activity.
    • This biochemical alteration is a key factor contributing to retinal degeneration and vision loss in affected dogs.

    Related Experiment Videos