Related Experiment Video

Updated: Feb 6, 2026

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
10:54

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Published on: April 4, 2018

16.0K

Reversine inhibits Colon Carcinoma Cell Migration by Targeting JNK1

Mohamed Jemaà1,2, Yasmin Abassi3, Chamseddine Kifagi4

  • 1Department of Laboratory Medicine, Translational Cancer Research, Lund University, Lund, 22381, Sweden. jemaamohamed@gmail.com.

Scientific Reports
|August 9, 2018
PubMed

Insights

The small molecule Reversine inhibits colon cancer cell migration by targeting the JNK1 pathway. This study identifies Reversine as a potential new drug to combat colorectal cancer metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer-related death, with metastasis driving patient mortality.
  • Novel therapeutic strategies are crucial to inhibit the dissemination and metastasis of colorectal tumor cells.

Purpose of the Study:

  • To investigate the anti-metastatic effects of the small molecule Reversine on human colon carcinoma cells.
  • To identify the molecular pathway targeted by Reversine in the context of cancer cell migration.

Main Methods:

  • In vitro migration assays using human colon carcinoma cells.
  • Kinase assays and bioinformatics analysis to identify the targeted signaling pathway.
  • Gene knockdown and pharmacological inhibition of specific kinases.
  • In vivo xenograft studies to assess metastatic potential.

Main Results:

  • Reversine significantly reduced the migration potential of colon carcinoma cells in vitro.
  • The c-Jun N-terminal kinase (JNK) cascade was identified as the primary pathway inhibited by Reversine.
  • JNK1, but not JNK2, was identified as a key downstream effector in mediating Reversine's anti-migratory effects.
  • Inhibition of JNK signaling in vivo reduced the metastatic potential of colon cancer cells.

Conclusions:

  • Reversine demonstrates anti-metastatic properties by inhibiting JNK1-mediated cancer cell migration.
  • This study highlights the differential roles of JNK isoforms in cancer metastasis.
  • Reversine is proposed as a potential novel therapeutic agent for colorectal cancer treatment.

More Related Videos

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.4K
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells07:48

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Published on: October 9, 2013

21.4K

Related Concept Videos

Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.6K
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.8K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.2K
The Colonization of Land02:22

The Colonization of Land

Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
37.7K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.9K