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Simultaneous Optogenetics and Cellular Resolution Calcium Imaging During Active Behavior Using a Miniaturized

Alice M Stamatakis1, Mike J Schachter1, Srishti Gulati1

  • 1Inscopix Inc., Palo Alto, CA, United States.

Frontiers in Neuroscience
|August 9, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel integrated microscope for simultaneous neural circuit manipulation and calcium imaging in mice. This tool enables direct investigation of how neural dynamics influence behavior, advancing brain research.

Keywords:
GRIN lensescalcium imagingfreely behavingneurocircuitsoptogenetics

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Area of Science:

  • Neuroscience
  • Optical Engineering
  • Bioengineering

Background:

  • Understanding brain function relies on precise neural circuit monitoring and manipulation.
  • Optical techniques like calcium imaging and optogenetics have significantly advanced neuroscience.
  • Combining these methods offers a direct way to link neural activity to behavior.

Purpose of the Study:

  • To develop a miniaturized microscope for simultaneous optogenetic manipulation and cellular-resolution calcium imaging.
  • To assess and minimize optical and biological crosstalk between imaging and manipulation.
  • To demonstrate the utility of the integrated microscope in probing circuit-behavior relationships.

Main Methods:

  • Development of a miniaturized integrated microscope with dual LEDs for calcium imaging and optogenetics.
  • Utilized specific excitation filters (435-460 nm for imaging, 590-650 nm for optogenetics).
  • Tested for biological and optical crosstalk, and applied the system to the basolateral amygdala (BLA)-to-nucleus accumbens (NAc) circuit in freely behaving mice.

Main Results:

  • The integrated microscope achieved simultaneous imaging and manipulation with minimal crosstalk, particularly the optogenetic LED not affecting calcium imaging.
  • Demonstrated significant behavioral and cellular calcium responses upon optogenetic modulation of the BLA-to-NAc circuit.
  • Validated the system's capability to investigate causal relationships between circuit activity and behavior.

Conclusions:

  • The developed integrated microscope is a valuable tool for neuroscience research.
  • It enables routine investigation of the causal impact of neural circuit manipulation on network dynamics and behavior.
  • This technology facilitates a deeper understanding of brain function in freely behaving animals.