Related Experiment Video
Updated: Feb 6, 2026
08:58
Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
Published on: October 17, 2025
678
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors
Alison G Tebo1, Frederico M Pimenta1, Martha Zoumpoulaki1
1PASTEUR, Département de Chimie, École Normale Supérieure , PSL University, Sorbonne Université , CNRS, 75005 Paris , France.
ACS Chemical Biology
|August 9, 2018
Summary
Researchers developed circularly permuted FAST (cpFAST) variants for optical biosensors. These novel cpFAST reporters enable real-time imaging of intracellular analytes like calcium in living cells.
Area of Science:
- Biotechnology
- Molecular Biology
- Cellular Imaging
Background:
- Fluorescent reporters are crucial for optical biosensors used in live-cell imaging of intracellular analytes.
- Existing biosensor designs require optimization for enhanced sensitivity and specificity.
Discussion:
- This study introduces circularly permuted variants of FAST (cpFAST) as a novel reporting module for biosensors.
- cpFAST variants enable analyte-specific fluorescence activation by linking ligand binding to fluorogen activation.
- This modular design facilitates the creation of "plug-and-play" biosensors.
Key Insights:
- Circularly permuted FAST (cpFAST) variants were successfully developed and characterized.
- These cpFAST variants serve as effective reporting modules in genetically encoded biosensors.
- The study demonstrates the creation of multicolor biosensors for real-time intracellular Ca2+ imaging in mammalian cells.
Outlook:
- cpFAST variants hold significant potential for advancing optical biosensor development.
- Future applications may include imaging a wider range of intracellular analytes and in various biological systems.
- This work paves the way for more sophisticated live-cell imaging tools in biological research.
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