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Cell paintballing using optically targeted coacervate microdroplets.

James P K Armstrong1,2,3, Sam N Olof1,2,4, Monika D Jakimowicz1,2,3,4

  • 1Bristol Centre for Functional Nanomaterials , University of Bristol , BS8 1FD , UK.

Chemical Science
|August 10, 2018
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Summary
This summary is machine-generated.

We developed "cell paintballing" to precisely deliver biomolecules to single cells. This method uses coacervate microdroplets assembled by holography for efficient cell functionalization.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Precise delivery of biomolecular payloads to individual cells is crucial for understanding cellular functions and developing new therapies.
  • Existing methods often lack spatial precision or require complex procedures.

Purpose of the Study:

  • To develop a novel technology for highly spatially precise, directed delivery of biomolecular payloads to individual cells.
  • To demonstrate the efficacy of coacervate microdroplets for cell functionalization.

Main Methods:

  • Active sequestration of proteins, oligonucleotides, or molecular dyes into coacervate microdroplets.
  • Delivery of these microdroplets to specific regions of stem cell membranes using a dynamic holographic assembler.
  • Observation of spontaneous coacervate microdroplet-membrane fusion.

Main Results:

  • Achieved directed delivery of biomolecular payloads to individual cells with high spatial precision.
  • Demonstrated facile preparation and high sequestration efficiency of coacervate microdroplets.
  • Confirmed inherent membrane affinity and spontaneous fusion of microdroplets with cell membranes.

Conclusions:

  • The developed
  • cell paintballing
  • technology offers a highly advantageous method for spatially-directed cell functionalization.
  • This approach has potential applications in single-cell stimulation, transfection, and differentiation.