Related Experiment Video
Updated: Feb 6, 2026
07:23
Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host
Published on: September 14, 2021
2.7K
Unconventional RNA-binding proteins step into the virus-host battlefront
Manuel Garcia-Moreno1, Aino I Järvelin1, Alfredo Castello1
1Department of Biochemistry, University of Oxford, Oxford, UK.
Wiley Interdisciplinary Reviews. RNA
|August 10, 2018
Summary
Cellular RNA-binding proteins (RBPs) are vital in viral infections. Recent studies reveal hundreds of RBPs with unconventional domains, offering new therapeutic targets for antiviral strategies.
Area of Science:
- Molecular Biology
- Virology
- Biochemistry
Background:
- Cellular RNA-binding proteins (RBPs) play critical roles in viral infections.
- Traditional research focused on RBPs with classical RNA-binding domains (RBDs).
- Recent proteome-wide studies identified numerous RBPs with unconventional RBDs.
Purpose of the Study:
- To compare the experimentally determined RBP census with gene ontology terms and literature.
- To identify RBPs with known links to viral infections.
- To explore the potential of unconventional RBPs in antiviral therapies.
Main Methods:
- Comparative analysis of RBP census data.
- Literature review and gene ontology term enrichment.
- Review of pioneering examples of unorthodox RBPs in viral infections.
Main Results:
- 472 proteins were identified with documented links to viruses.
- Unconventional RBDs include protein-protein interaction platforms, enzymatic cores, and disordered regions.
- These proteins have diverse potential roles in viral infection.
Conclusions:
- A significant number of RBPs, particularly those with unconventional domains, are implicated in viral infections.
- Understanding these unorthodox RBPs is crucial for comprehending virus-host interactions.
- Unconventional RBPs represent promising targets for novel host-based antiviral therapies.
Keywords:
RBPomeRNARNA interactome captureRNA metabolismRNA-binding domainRNA-binding proteininfectiontranslationvirusMore Related Videos
11:49A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Published on: August 21, 2018
7.0K
12:24PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
Published on: July 2, 2010
54.2K
Related Concept Videos
00:50What are Viruses?
128.3K
Overview
128.3K
02:36RNA Polymerase II Accessory Proteins
11.0K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.0K
01:29Viruses with RNA Genomes
935
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
935
00:49Ligand Binding and Linkage
5.6K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.6K
01:49Conserved Binding Sites
5.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.2K
02:24Regulation of Expression Occurs at Multiple Steps
26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K