[Study of Yiqi Chutan Formula in Suppressing A549 Lung Cancer by Regulating Unfolded Protein Response]

To study the inhibition and mechanism on lung cancer A549 cells xenografts in BALB / c nude mice.

40 BALB / c mice were selected to establish lung cancer xenografts models with human lung adenocarcinoma A549 cells,which were randomized into model group,cisplatin group( 0. 002 g / kg), Yiqi Chutan Formula low-dose group( 3. 0 g / kg),Yiqi Chutan Formula high-dose group( 6. 0 g / kg) and combination group[Yiqi Chutan Formula 6. 0( g / kg) / cisplatin 0. 002( g / kg) ], with eight mice in each group. Yiqi Chutan Formula was given once a day with 0. 2 m L from 8th day after modeling,and successive medication for14 days. After given cisplatin with 0. 2 m L by intraperitoneal injection once a day in 17 th day,and modeling for 5 days. The tumor weight and tumor volume were detected. And the tumor inhibitory rate were calculated. The expression of CNX and XBP1 was detected by immunohistochemistry,Western blotting and and real-time quantitative PCR.

Compared with the model group, the tumor weight and the tumor volume were lower in cisplatin group, Yiqi Chutan Formula high-dose group and combination group( P < 0. 01);and the combination group was better than the cisplatin group( P < 0. 01). The results of immunohistochemistry, Western blotting and real-time quantitative PCR showed that, compared with the model group, the expression of CNX and XBP1 were lower in cisplatin group, Yiqi Chutan Formula high-dose group and combination group( P < 0. 01),and the combination group was better than the cisplatin group( P < 0. 05 or P < 0. 01).

Yiqi Chutan Formula can inhibit A549 lung cancer cells in BALB/c nude mice. Combined with chemotherapy drugs, the killing effect on lung cancer can be enhanceed. Its mechanism may be related to down-regulating CNX and XBP1 to inhibit the unfolded protein response to lead to apoptosis.