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Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

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Cells Adhering to 3D Vertical Nanostructures: Cell Membrane Reshaping without Stable Internalization.

Michele Dipalo¹, Allister F McGuire², Hsin-Ya Lou²

¹Istituto Italiano di Tecnologia , Genoa 16163 , Italy.

|August 10, 2018

Summary

Cellular membranes conform to most 3D nanostructures without penetration. Spontaneous membrane penetration by vertical nanostructures is rare, maintaining cellular integrity and guiding biointerface design.

Keywords:

3D nanostructures biointerface cell cross-section cell membrane deformation cell penetration electroporation

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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

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Published on: July 23, 2010

Area of Science:

Biophysics
Materials Science
Nanotechnology

Background:

The cellular membrane-nanostructure interface is crucial for biological processes and biomedical device design.
Understanding cellular membrane behavior with nanoscale objects remains incomplete.
Artificial biointerfaces require knowledge of membrane mechanics at the nanoscale.

Purpose of the Study:

To characterize the mechanical stability of cellular membranes interacting with high-aspect-ratio 3D vertical nanostructures.
To investigate the conditions and frequency of nanostructure penetration into cell membranes.
To provide insights into cellular membrane deformation and integrity upon nanoscale interaction.

Main Methods:

Multifaceted characterization of cellular membrane mechanical stability.
Analysis of membrane interaction with various 3D vertical nanostructures.
Evaluation of nanostructure penetration and intracellular coupling formation.

Main Results:

Cellular membranes largely conform to nanostructures without compromising integrity.
Spontaneous penetration of vertical nanostructures into cell membranes is infrequent and condition-dependent.
The cell membrane demonstrates robust adaptability to diverse nanostructure shapes.

Conclusions:

Cellular membranes exhibit significant mechanical resilience against 3D nanostructures.
Nanostructure design for biointerfaces should consider the membrane's conforming ability rather than solely penetration.
Further research can optimize bio-nanostructure interactions for advanced biomedical applications.