Related Experiment Video
Updated: Feb 6, 2026

Author Spotlight: Investigating the Potential of Chinese Herbal Medicinal Active Dioscin in Treating IgA Nephropathy
Published on: October 13, 2023
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients
Colin Reily1, Dana V Rizk1, Bruce A Julian1,2
1University of Alabama at Birmingham, Department of Medicine, Birmingham, AL, USA.
Aims:
IgA nephropathy, the most common primary glomerulonephritis worldwide, is characterized by glomerular deposition of galactose-deficient IgA1 and elevated serum levels of this IgA1 glycoform. Current ELISA methods lack sensitivity to assess galactose deficiency using small amounts of IgA1, which limits studies in primary cells due to modest IgA1 production in isolated peripheral-blood lymphocytes.
Methods:
Lectin from Helix pomatia was conjugated to biotin or acridinium ester and used in ELISA to detect galactose deficiency of IgA1 using small amounts of IgA1.
Results:
Lectin conjugated to acridinium had an approximately a log-fold increased sensitivity compared with biotin-labeled lectin.
Conclusions:
The new method of using lectin from Helix pomatia conjugated to acridinium increased assay sensitivity, allowing future mechanistic studies with cultured primary cells.
More Related Videos
Related Concept Videos
Mechanistic Models: Overview of Compartment Models
Bioavailability Study Design: Healthy Subjects Versus Patients
Primary Active Transport
Mechanistic Models: Compartment Models in Individual and Population Analysis
Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving
In individual population analyses, different algorithms are employed, such as Cauchy's method, which uses a...
Primary and Secondary Growth in Roots and Shoots

