Organic matter protection by kaolinite over bio-decomposition as suggested by lignin and solvent-extractable lipid...

The formation of organo-mineral complexes is essential in organic matter (OM) stabilization. However, limited studies have been conducted to systematically examine the mineral influence on the decomposition of plant residuals at a molecular level. In this study, pine needles and chestnut leaves were mixed with kaolinite at the weight ratio of 5:1. The controls were plant tissues without kaolinite. All the samples were incubated in the laboratory for one year. Molecular markers, including lignin-derived phenols (e.g. Vanilly units, syringyl units and cinnamyl units) and solvent-extractable lipids (e.g. n-alkanoic acid, n-alkanols and n-alkanes), were analyzed. The concentrations of lignin-derived phenols and lipid compounds were higher in the presence of kaolinite than without kaolinite. Lower degradation indexes, such as (Ad/Al)_V (ratio of vanillic acid to vanillin) and CPI (carbon preference index of n-alkanoic acid and n-alkanes), were found in the kaolinite system. These results indicate that kaolinite reduced the OM decomposition. The addition of kaolinite also stabilized some carbohydrates from plants. Furthermore, the degradation of OM led to the generation of persistent free radicals, indicated by electron paramagnetic resonance (EPR) signals. The EPR signals were higher with than without kaolinite. We hypothesize that the adsorption of semiquinone or quinone radicals on kaolinite may limit their reaction with other OM moieties and thus extended their lifetimes. In addition to embedding OM in soil aggregates, our results provide direct evidence of another mineral protective mechanism of soil OM.