Related Experiment Video
Updated: Feb 6, 2026

Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung
Published on: January 31, 2025
pre-B cell colony enhancing factor negatively regulates Na+ and fluid transport in lung epithelial cells
Qiao Fang1, Miaomiao You1, Weichang Xu1
1Department of Cardiac Macrovascular Surgery, The Second Affiliated Hospital of Nanchang University Nanchang 330006, China.
Abstract:
This study was undertaken to investigate the effect of pre-B cell colony enhancing factor (PBEF) on Na+ and fluid transport in lung epithelial cells.
Methods:
Type 1 and 2 cells were isolated from lung epithelium. After hypoxia reoxygenation treatment, the primary cell cultures were transfected with a plasmid over-expressing PBEF. Sodium-potassium ATPase (NKA), epithelial sodium channel (ENaC), type I cell marker rT140, surfactant protein (SP) and PBEF protein were analyzed at mRNA and protein levels using PCR and Western blot analysis. Immunofluorescence assays showed type 1 and 2 cells were successfully isolated. After the transfection with PBEF over-expression vector, PBEF and RTI40 levels were increased, while ENaC and SP as well as NKA, were decreased in both cells. It is clear that PBEF negatively regulates the expression of ENaC and NKA in the Na+ and fluid transport in lung epithelial cells.
Related Concept Videos
Negative Regulator Molecules
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Regulated mRNA Transport
Regulated mRNA Transport
pH Regulation in Cells
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Transcription Factors

