Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Predicting Molecular Geometry02:27

Predicting Molecular Geometry

46.0K
VSEPR Theory for Determination of Electron Pair Geometries
46.0K
Prediction Intervals01:03

Prediction Intervals

3.4K
The interval estimate of any variable is known as the prediction interval. It helps decide if a point estimate is dependable.
However, the point estimate is most likely not the exact value of the population parameter, but close to it. After calculating point estimates, we construct interval estimates, called confidence intervals or prediction intervals. This prediction interval comprises a range of values unlike the point estimate and is a better predictor of the observed sample value, y. 
3.4K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
End Point Prediction: Gran Plot01:07

End Point Prediction: Gran Plot

1.2K
A Gran plot is used to predict the equivalence volume or endpoint of a potentiometric or acid-base titration without reaching the endpoint. Typically, titration data is collected as a function of the titrant's volume up to a point less than the equivalence volume and then transformed into a linear format. The straight line is extended to the x-axis, indicating the necessary titrant volume to achieve the equivalence point.
For potentiometric titration, the Gran plot is created by plotting...
1.2K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.4K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.4K
Protein and Protein Structure02:15

Protein and Protein Structure

88.2K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
88.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioluminescent label-free molecular switch for SARS-CoV-2 detection.

Analytica chimica acta·2026
Same author

Oral yeast-displayed SARS-CoV-2 spike protein vaccine enhanced by trimerization and flagellin peptide.

Scientific reports·2026
Same author

Transcriptomic and physiological analysis of Dunaliella salina under sistan deep water in Iran.

Scientific reports·2026
Same author

The Role of the Golgi Apparatus in Neurodegeneration.

Sub-cellular biochemistry·2026
Same author

Femtogram-level VEGF detection via PEG-directed gold nanostructured electrochemical immunosensor.

Scientific reports·2025
Same author

Liposomal Cancer Drug Database (LCDD): a comprehensive resource for liposome research in cancer therapy and diagnosis.

Database : the journal of biological databases and curation·2025

Related Experiment Video

Updated: Feb 6, 2026

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K

afpCOOL: A tool for antifreeze protein prediction.

Morteza Eslami1, Ramin Shirali Hossein Zade2, Zeinab Takalloo3

  • 1Department of Computer Engineering, Arak University, Arak, Iran.

Heliyon
|August 11, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method using machine learning to identify antifreeze proteins (AFPs), which protect organisms from freezing. This novel approach, afpCOOL, accurately predicts AFPs and outperforms existing methods.

Keywords:
BiochemistryBioinformaticsComputational biologyComputer scienceMathematical biosciences

More Related Videos

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
08:46

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

9.6K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.8K

Related Experiment Videos

Last Updated: Feb 6, 2026

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
08:46

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

9.6K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.8K

Area of Science:

  • Biochemistry
  • Computational Biology
  • Molecular Biology

Background:

  • Cold-adapted organisms produce antifreeze proteins (AFPs) that inhibit ice crystal growth, preventing cellular freezing.
  • AFPs have significant applications in agriculture, including enhancing plant freeze tolerance and creating cold-hardy crops via transgenic technology.
  • Identifying AFPs is challenging due to sequence and structural diversity among these proteins.

Purpose of the Study:

  • To propose a novel computational method for identifying antifreeze proteins (AFPs).
  • To evaluate the proposed method's performance against existing state-of-the-art techniques.
  • To determine the most effective feature types for AFP prediction.

Main Methods:

  • Utilized a supportive vector machine (SVM) algorithm for protein classification.
  • Incorporated four distinct feature types, including physicochemical descriptors, to represent protein characteristics.
  • Validated the method on two benchmark datasets and an independent test set.

Main Results:

  • The proposed SVM-based method demonstrated strong performance in predicting AFPs.
  • The afpCOOL tool achieved superior accuracy compared to current state-of-the-art methods on an independent test dataset.
  • Physicochemical descriptors were identified as the most significant features for accurate AFP detection.

Conclusions:

  • The developed method provides a robust and accurate approach for identifying antifreeze proteins.
  • The afpCOOL tool offers a user-friendly solution for predicting AFPs across various operating systems.
  • This research advances the understanding and application of AFPs in fields like agriculture and cryopreservation.