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LLC tumor cells-derivated factors reduces adipogenesis in co-culture system

Magno Alves Lopes1, Felipe Oliveira Franco1, Felipe Henriques2

  • 1Laboratory of Adipose Tissue Biology, Center for Integrated Biotechnology, University of Mogi das Cruzes, Mogi das Cruzes, São Paulo, Brazil.

Heliyon
|August 11, 2018
PubMed

Insights

Cancer cachexia involves weight loss. This study shows Lewis Lung Carcinoma cells inhibit fat cell (adipocyte) differentiation and promote apoptosis, impacting the adipogenic process.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Cancer cachexia (CC) is a complex syndrome characterized by progressive weight loss.
  • Early stages of CC involve down-regulation of adipogenic and lipogenic genes in adipose tissue (AT).
  • Tumor cell products are suspected to influence adipose tissue remodeling during cachexia.

Purpose of the Study:

  • To investigate the impact of Lewis Lung Carcinoma (LLC) cells on the adipogenic capacity of 3T3-L1 pre-adipocytes.
  • To elucidate the molecular mechanisms by which tumor cells affect adipogenesis in a co-culture system.

Main Methods:

  • Co-culture of Lewis Lung Carcinoma (LLC) cells with 3T3-L1 pre-adipocytes.
  • Assessment of lipid accumulation during adipocyte differentiation.
  • Analysis of key adipogenic gene expression (PPARγ, C/EBPα, adiponectin, perilipin, FABP4).
  • Evaluation of Caspase-3, IL-6, and TNF-α expression.

Main Results:

  • LLC cells significantly reduced lipid accumulation in differentiating 3T3-L1 cells.
  • Early down-regulation of PPARγ and C/EBPα was observed, followed by late-acting adipogenic genes.
  • Increased Caspase-3 expression indicated enhanced apoptosis.
  • Upregulation of pro-inflammatory cytokines IL-6 and TNF-α was noted.

Conclusions:

  • Secretory products from LLC tumor cells impair adipocyte differentiation.
  • LLC cells promote apoptosis and inflammation in the co-culture system.
  • This study demonstrates the inhibition of the adipogenic process by LLC cells in vitro.
Keywords:
Cancer researchCell biologyMolecular biology

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