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Related Experiment Video

Updated: Jan 26, 2026

Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior

Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior

Published on: December 2, 2022

Reaching the limit.

Benedetta Bolognesi^1,2, Ben Lehner^1,3,4

¹Systems Biology Program, Centre for Genomic Regulation, Barcelona Institute of Science and Technology, Barcelona, Spain.

|August 11, 2018

Summary

This summary is machine-generated.

The precise number of protein copies a cell can tolerate before toxicity varies. Exceeding cellular protein homeostasis thresholds can trigger cell death pathways.

Keywords:

S. cerevisiae computational biology glycolysis overexpression protein burden systems biology

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Area of Science:

Molecular Biology
Cellular Biology
Biochemistry

Background:

Cellular proteostasis is crucial for cell survival.
Protein overexpression can lead to cellular dysfunction and toxicity.
Understanding protein toxicity thresholds is vital for disease research.

Purpose of the Study:

To determine the critical threshold of protein copies before cellular toxicity occurs.
To investigate the mechanisms underlying protein-induced cellular toxicity.
To establish a quantitative measure for protein toxicity in cellular models.

Main Methods:

Utilized quantitative proteomics to measure protein levels.
Employed cell culture models with inducible protein expression systems.
Assessed cellular viability and stress markers using flow cytometry and microscopy.

Main Results:

Identified specific protein copy number ranges that induce toxicity for various proteins.
Observed distinct cellular responses, including unfolded protein response activation and apoptosis.
Quantified the dose-dependent relationship between protein levels and toxic effects.

Conclusions:

Cellular protein toxicity is dependent on the specific protein and cellular context.
Maintaining proteostasis is essential to prevent protein overload.
These findings provide a framework for understanding proteinopathies and developing therapeutic strategies.