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Electrochemiluminescence Assays for Human Islet Autoantibodies
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Human monoclonal autoantibodies that react with both pancreatic islets and thyroid

C Garzelli, F E Taub, M C Jenkins

    The Journal of Clinical Investigation
    |May 1, 1986
    PubMed

    Transformation of human peripheral blood lymphocytes with Epstein-Barr virus and rapid screening on rat insulinoma cells by an enzyme-linked immunosorbent assay were used to identify monoclonal autoantibodies that reacted with human pancreatic islets. Six such monoclonal autoantibodies were isolated and cloned. All six also were found to react with human thyroid. It is concluded that lymphocytes able to make autoantibodies that react with both the pancreas and thyroid are common in the human B cell repertoire.

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