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Related Concept Videos

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

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Affect plays a crucial role in shaping interpersonal evaluations and perceptions. Emotions influence how individuals judge and respond to others, often determining whether interactions are viewed positively or negatively. This effect can manifest directly through interactions with the person in question or indirectly via associations with unrelated emotional experiences.Direct Effects of Affect on AttractionAffect directly influences interpersonal attraction when a person’s behavior...
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Positive affect significantly influences cognitive processes, including evaluation, memory, creativity, and social judgments. Compared to negative affect, positive emotional states promote more favorable interpretations of stimuli, cognitive flexibility, and heuristic processing. These effects highlight emotions' powerful role in shaping how individuals perceive, remember, and interact with the world.Influence on Evaluation and AttributionWhen individuals experience positive affect, they are...
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Related Experiment Video

Updated: Feb 6, 2026

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow
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F2-isoprostanes affect macrophage migration and CSF-1 signalling.

J M Proudfoot1, M W Murrey2, S McLean2

  • 1School of Biomedical Sciences, University of Western Australia, Royal Perth Hospital Medical Research Foundation, GPO Box X2213, Perth, Western Australia 6847, Australia.

Free Radical Biology & Medicine
|August 11, 2018
PubMed
Summary

15-F2t-isoprostane (15-F2t-IsoP) stimulates macrophage migration towards CSF-1, despite inhibiting cell adhesion. This isoprostane impacts macrophage signaling pathways, influencing their role in atherosclerosis development.

Keywords:
CSF-1F(2)-isoprostanesMacrophage migrationMacrophagesOxidized lipids

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Oxidative stress, indicated by F2-isoprostanes (F2-IsoP), is linked to atherosclerosis.
  • Macrophages are key players in atherosclerosis, and their migration is crucial.
  • Colony stimulating factor-1 (CSF-1) is vital for macrophage function and present in atherosclerotic plaques.

Purpose of the Study:

  • To investigate the effect of 15-F2t-isoprostane (15-F2t-IsoP) on macrophage migration.
  • To explore the underlying signaling pathways involved in 15-F2t-IsoP-mediated macrophage responses.
  • To determine the role of specific adhesion kinases in 15-F2t-IsoP's effects on macrophages.

Main Methods:

  • Macrophage cell lines (BAC1.2F5) and primary bone marrow-derived macrophages were used.
  • Cell migration was assessed using Boyden chambers and wound healing assays.
  • Signaling pathways were analyzed via Western blotting, and gene silencing (siRNA) was employed for FAK and Pyk2.

Main Results:

  • 15-F2t-IsoP dose-dependently inhibited BAC1.2F5 macrophage spreading and adhesion but stimulated migration towards CSF-1.
  • 15-F2t-IsoP decreased Akt and Rictor phosphorylation while increasing FAK, Pyk2, and paxillin phosphorylation.
  • Pyk2 silencing counteracted 15-F2t-IsoP-induced reduction in cell area and phospho-paxillin adhesions, with a trend towards reduced migration.

Conclusions:

  • 15-F2t-IsoP modulates macrophage adhesion and migration, impacting key signaling pathways.
  • The findings highlight a novel role for 15-F2t-IsoP in macrophage behavior relevant to atherosclerosis.
  • Pyk2 appears to play a significant role in mediating 15-F2t-IsoP's effects on macrophage adhesion and migration.