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Published on: October 21, 2022
Fabrication of a Urea Biosensor for Real-Time Dynamic Fluid Measurement
Kyunghee Kim1,2,3, Jeongeun Lee4,5,6, Bo Mi Moon7
1Cooperative Course of Nano-Medical Device Engineering, Hallym University, Chuncheon 24252, Korea. facility.hee@gmail.com.
Abstract:
In this study, a portable urea sensor that monitors the urea concentration in flow conditions was fabricated. We propose an electrochemical sensor that continually measures the urea concentration of samples flowing through it at a constant flow rate in real time. For the electrochemical sensing, a porous silk fibroin membrane with immobilized urease was mounted in a polydimethylsiloxane (PDMS) sensor housing. The fabricated urea sensor elicited linear current⁻concentration characteristics in the clinically significant concentration range (0.1⁻20 mM) based on peritoneal dialysis. The sensor maintained the linear current⁻concentration characteristics during operation in flow conditions.
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