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Updated: Feb 6, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Published on: August 16, 2012
Dynamic Tomographic Reconstruction of Deforming Volumes
Clément Jailin1, Stéphane Roux2
1LMT (ENS Paris-Saclay/CNRS/University Paris-Saclay), 61 avenue du Président Wilson, F-94235 Cachan, France. clement.jailin@ens-paris-saclay.fr.
Abstract:
The motion of a sample while being scanned in a tomograph prevents its proper volume reconstruction. In the present study, a procedure is proposed that aims at estimating both the kinematics of the sample and its standard 3D imaging from a standard acquisition protocol (no more projection than for a rigid specimen). The proposed procedure is a staggered two-step algorithm where the volume is first reconstructed using a "Dynamic Reconstruction" technique, a variant of Algebraic Reconstruction Technique (ART) compensating for a "frozen" determination of the motion, followed by a Projection-based Digital Volume Correlation (P-DVC) algorithm that estimates the space/time displacement field, with a "frozen" microstructure and shape of the sample. Additionally, this procedure is combined with a multi-scale approach that is essential for a proper separation between motion and microstructure. A proof-of-concept of the validity and performance of this approach is proposed based on two virtual examples. The studied cases involve a small number of projections, large strains, up to 25%, and noise.
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