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Updated: Feb 6, 2026

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Published on: October 1, 2012
Detection of ADP-Ribosylating Bacterial Toxins
Chen Chen1, Joseph T Barbieri2
1Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, USA.
Abstract:
Many bacterial toxins catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD) to a host protein. Greater than 35 bacterial ADP-ribosyltransferase toxins (bARTTs) have been identified. ADP-ribosylation of host proteins may be specific or promiscuous. Despite this diversity, bARTTs share a common reaction mechanism, three-dimensional active site structure, and a conserved active site glutamic acid. Here, we describe how to measure the ADP-ribosylation of host proteins as purified proteins or within a cell lysate.
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