Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Structural and functional studies of insertion element IS200.

S Lam, J R Roth

Journal of Molecular Biology

|January 20, 1986

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Emerging roles of S100 proteins in podocyte biology and disease: A mini review.

Biochimica et biophysica acta. Proteins and proteomics·2026

Same author

Novel blood signature for hepatocellular carcinoma screening.

ESMO gastrointestinal oncology·2026

Same author

Tumor and blood B-cell abundance outperforms established immune checkpoint blockade response prediction signatures in head and neck cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Same author

Lung cancer research and treatment: global perspectives and strategic calls to action.

Annals of oncology : official journal of the European Society for Medical Oncology·2024

Same author

A new variant of gallbladder duplication mimicking a choledochal cyst: stepwise management of an unexpected surgical finding.

Annals of the Royal College of Surgeons of England·2024

Same author

Multi-breed host rumen epithelium transcriptome and microbiome associations and their relationship with beef cattle feed efficiency.

Scientific reports·2023

Same journal

Clinical inflammasome biomarkers: Progress and prospects.

Journal of molecular biology·2026

Same journal

Biologically Relevant, Cationic Residues in Human Rhinovirus Stabilize Capsid-Bound RNA Duplexes, and Restrict Capsid Flexibility.

Journal of molecular biology·2026

Same journal

Cryo-EM structures of phage T4 infection intermediate.

Journal of molecular biology·2026

Same journal

A classic fold with a twist: Structural architecture of Dhillonvirus phage Bas18.

Journal of molecular biology·2026

Same journal

Tesorai Search: cloud-based database search engine boosts identifications for mass spectrometry proteomics with a pretrained peptide-spectrum deep-learning model.

Journal of molecular biology·2026

Same journal

Characterization of diverse functions of NRF1 nuclear localization sequence.

Journal of molecular biology·2026

See all related articles

The IS200 insertion element

Area of Science:

Molecular Biology
Genetics

Background:

Insertion sequences (IS) are mobile genetic elements.
IS200 is a well-characterized insertion element.

Purpose of the Study:

To determine the nucleotide sequence of the IS200 insertion element.
To investigate the transcriptional effects of IS200 insertion.

Main Methods:

DNA sequencing
Genetic analysis

Main Results:

Partial nucleotide sequence of IS200 determined.
IS200 insertion strongly blocks host transcription.
A transcription termination signal identified near the right end of IS200.
A promoter identified within IS200, forming an internal transcription unit.

Related Experiment Videos

Conclusions:

IS200 possesses regulatory sequences (promoter and terminator).
These sequences allow IS200 to function as an independent transcription unit.
IS200's ability to block transcription is linked to its terminal sequences.