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Isolation of a receptor protein involved in attachment of human rhinoviruses

J E Tomassini, R J Colonno

    Journal of Virology
    |May 1, 1986
    PubMed

    Insights

    Researchers identified the cellular receptor for major group human rhinoviruses using a specific monoclonal antibody. This 90-kilodalton protein is crucial for rhinovirus attachment to cells.

    Area of Science:

    • Virology
    • Cell Biology
    • Immunology

    Background:

    • Human rhinoviruses are classified into major and minor groups based on receptor usage.
    • A specific monoclonal antibody was developed that blocks major group rhinovirus attachment.

    Purpose of the Study:

    • To isolate and characterize the cellular receptor for major group human rhinoviruses.
    • To confirm the functional role of the isolated receptor in viral attachment.

    Main Methods:

    • Utilized a monoclonal antibody to isolate the cellular receptor.
    • Developed a radioimmunoassay for receptor detection during purification.
    • Purified the receptor protein using immunoaffinity chromatography.
    • Analyzed protein molecular weight via SDS-PAGE and gel filtration.

    Main Results:

    • Isolated a solubilized receptor with an apparent molecular weight of 440,000 Da.
    • Purified a 90,000 Da receptor protein.
    • Antiserum against the purified protein blocked major group rhinovirus attachment.
    • The 90 kDa protein plays a functional role in viral attachment.

    Conclusions:

    • The 90 kDa protein is the cellular receptor for major group human rhinoviruses.
    • This receptor is essential for viral entry and can be targeted for therapeutic intervention.

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