Trypsin electrochemical sensing using two-dimensional molecularly imprinted polymers on 96-well microplates

Area of Science:

• Analytical Chemistry
• Materials Science
• Biotechnology

Background:

• Molecular imprinting creates artificial receptors mimicking antibody functions for separation and sensing.
• Developing efficient and sensitive detection methods is crucial for biological analysis.

Purpose of the Study:

• To introduce a new sensing method combining 2D-MIP films with CuO NPs for signal amplification.
• To construct and evaluate a trypsin-imprinted polymer-based electrochemical sensor for biological fluid analysis.

Main Methods:

• Fabrication of a two-dimensional molecularly imprinted polymer (2D-MIP) film.
• Utilizing copper oxide nanoparticles (CuO NPs) for signal conversion and amplification.
• Development of a trypsin-imprinted polymer-based electrochemical sensor on 96-well microplates.

Main Results:

• The CuO labeling effectively monitored 2D-MIP thickness for optimal imprinting.
• The electrochemical sensor demonstrated a good dynamic response in the range of 0.5-500 ng/mL for trypsin.
• Successful detection of trypsin in fetal bovine serum using the developed imprinted polymer thin films.

Conclusions:

• The developed electrochemical sensors exhibit excellent specificity, fast kinetics, and high sensitivity.
• The low-cost, efficient sensor shows great potential for practical applications in biological analysis.
• This novel method provides a promising platform for sensitive and selective biomolecule detection.