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Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
Coacervation of resilin fusion proteins containing terminal functionalities
Wenwen Fang1, Nonappa2, Marika Vitikainen3
1Department of Bioproducts and Biosystems, Aalto University, Espoo, FI-00076, AALTO, Finland.
Abstract:
Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.
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