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Diallyl trisulfide regulates rat colonic smooth muscle contractions by inhibiting L-type calcium channel currents
Qincai Tang1, Haixia Ren1, Lin Yan1
1Department of Gastroenterology, Renmin Hospital of Wuhan University, 430060, Wuhan, Hubei Province, China; Hubei Key Laboratory of Digestive System Disease, 430060, Wuhan, Hubei Province, China.
Abstract:
Diallyl trisulfide (DATS) is an active organosulfide component of allicin and has several beneficial effects, including antimicrobial, antioxidant, cardioprotective and anticancer effects. Few studies have shown the modulatory effect of DATS on L-type calcium channels in rat colonic smooth muscle cells and colonic motility. To investigate the modulatory effect of DATS on L-type calcium channels in rat colonic smooth muscle and colonic contraction, L-type calcium channel currents were recorded, and colonic contractility in longitudinal and circular smooth muscle strips was measured. DATS attenuated L-type calcium channel currents without affecting steady-state activation or inactivation kinetics and inhibited the spontaneous contractions of both longitudinal and circular smooth muscle strips dose-dependently. In conclusion, DATS has an inhibitory effect on the contractions of colonic muscle strips that is related to its regulation of L-type calcium channels.
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