Removal of dichlorophenol by Chlorella pyrenoidosa through self-regulating mechanism in air-tight test environment

Microalgae are surprisingly efficient to remove pollutants in a hermetically closed environment, though its growth is inhibited in the absence of pollutants. The final pH, algal density, Chl-a content, and the removal efficiency of 2,4-dichlorophenol (2,4-DCP) by Chlorellar pyrenoidosa in a closed system were observed under different initial pH, lighting regimes, and various carbon sources. The optimal condition for 2,4-DCP removal was obtained, and adopted to observe the evolution of above items by domesticated and origin strains. The results showed that both respiration and photosynthesis participated in the degradation of 2,4-DCP, and caused the changes of pH. The photosynthesis seemed to increase the solution pH, while the respiration and the biodegradation of 2,4-DCP to decrease the solution pH. The domesticated strain achieved nearly 100% removal when initial concentrations of 2,4-DCP lower than 200 μg L^-1, due to providing a appropriate but narrow pH evolution range, mostly falling between 6.5 and 7.9. The research helps to understand the mechanism of biodegradation of chlorophenol compounds by green algae.