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Updated: Feb 6, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
A shotgun method for high throughput screening microcystins in Margarya melanioides on a triple quadrupole tandem
Shiyan Li1, Yiwei Cui2, Yang Wang3
1The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China; Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou 310012, China.
Abstract:
A selective and sensitive procedure for determining seven hazardous microcystins in Margarya melanioides, using tandem mass spectrometry, is presented. The product ion m/z 135.2 was selected as representative for precursor ion scanning and used to increase overall sensitivity of this shotgun method. The method was validated, and recoveries ranged from 69.3 to 87.9%, with the relative standard deviation less than 10%. Limits of detection and quantitation were less than 3.1 and 9.3 ng·mL-1, respectively. A total of 60 samples were tested using this method. 38 samples were found to be contaminated with microcystins, among which there are 31 samples whose estimated daily intake (EDI) was 0-7.8 times higher than the tolerable daily intake (TDI). This method would be useful for carrying out direct, ultra-rapid quantification of microcystins in foods, and is of significant interest for environmental and food safety applications.
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