Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.1K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.1K
Histone Modification02:32

Histone Modification

16.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.2K
Histone Modification02:32

Histone Modification

4.5K
4.5K
Mutations01:39

Mutations

94.5K
Overview
94.5K
Mutations01:35

Mutations

44.6K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
44.6K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

H2BE113K mutation promotes breast cancer metastasis through modulating chromatin dynamics.

Science advances·2026
Same author

Neoadjuvant therapy in primary liver cancer: From the missing piece to a new paradigm.

Hepatology communications·2026
Same author

Agreement between predictive equations and indirect calorimetry in estimating resting metabolic rate among free-living older adults with sarcopenia: A prospective observational study.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2026
Same author

Effectiveness of combined cognitive and exercise training versus single exercise training on cognition in people with Parkinson's disease: a systematic review and meta-analysis of randomized controlled trials.

Aging & mental health·2026
Same author

Calcium Dysregulation Promotes Glioma Progression by Inhibiting STAT3 Degradation Through Blocking Chaperone-Mediated Autophagy.

Journal of cellular and molecular medicine·2026
Same author

A pharmacovigilance data-driven approach to reveal high fatal adverse events following checkpoint immunotherapy.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 6, 2026

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

22.2K

Histone H3 Mutations in Cancer.

Yi Ching Esther Wan1,2, Jiaxian Liu1,2, Kui Ming Chan1,2

  • 11Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.

Current Pharmacology Reports
|August 14, 2018
PubMed
Summary

Histone H3 mutations, known as oncohistones, disrupt normal histone methylation and drive cancer development. Understanding these mutations offers new therapeutic targets for cancers like pediatric diffuse intrinsic pontine glioma.

Keywords:
CancerH3K27MH3K36MHistone mutations

More Related Videos

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

41.8K
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

11.6K

Related Experiment Videos

Last Updated: Feb 6, 2026

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Published on: November 30, 2018

22.2K
Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

41.8K
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
09:26

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Published on: March 26, 2017

11.6K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Oncology

Background:

  • Histone modifications, particularly methylation, are crucial for gene regulation and epigenetic information.
  • Alterations in chromatin-modifying enzymes and histone mutations are linked to cancer development.
  • Specific histone H3 mutations (H3K27M, H3K36M, H3G34V/R) impede histone methylation, acting as oncohistones.

Purpose of the Study:

  • To review the identification and mechanistic dissection of oncogenic histone H3 mutations.
  • To summarize the role of oncohistones in reprogramming the epigenome and driving cancer.
  • To highlight therapeutic targets discovered through studying H3K27M-driven oncogenesis.

Main Methods:

  • Review of existing literature on histone mutations and their functional consequences.
  • Analysis of the impact of specific histone H3 mutations on global histone methylation patterns.
  • Investigation into the epigenetic reprogramming and transcriptomic changes induced by oncohistones.

Main Results:

  • Histone H3 mutations result in amino acid substitutions near methylation target sites.
  • Mutant histones H3K27M and H3K36M cause global reduction in histone methylation.
  • Oncohistone expression reprograms the epigenome, leading to an oncogenic transcriptome.

Conclusions:

  • Histone H3 mutations are key drivers of oncogenesis through epigenetic dysregulation.
  • Understanding oncohistones provides insights into cancer mechanisms and potential therapies.
  • Targeting H3K27M-driven oncogenesis has yielded promising therapeutic strategies for pediatric DIPG.