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LipidFinder on LIPID MAPS: peak filtering, MS searching and statistical analysis for lipidomics
Eoin Fahy1, Jorge Alvarez-Jarreta2, Christopher J Brasher2
1Department of Bioengineering, University of California San Diego, San Diego, CA, USA.
Summary:
We present LipidFinder online, hosted on the LIPID MAPS website, as a liquid chromatography/mass spectrometry (LC/MS) workflow comprising peak filtering, MS searching and statistical analysis components, highly customized for interrogating lipidomic data. The online interface of LipidFinder includes several innovations such as comprehensive parameter tuning, a MS search engine employing in-house customized, curated and computationally generated databases and multiple reporting/display options. A set of integrated statistical analysis tools which enable users to identify those features which are significantly-altered under the selected experimental conditions, thereby greatly reducing the complexity of the peaklist prior to MS searching is included. LipidFinder is presented as a highly flexible, extensible user-friendly online workflow which leverages the lipidomics knowledge base and resources of the LIPID MAPS website, long recognized as a leading global lipidomics portal.
Availability And Implementation:
LipidFinder on LIPID MAPS is available at: http://www.lipidmaps.org/data/LF.
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