Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Proteolysis in quaking mouse brain and spinal cord.

W W McAlhaney, N L Banik, S Greenfield

Neurochemical Research

|February 1, 1986

Summary

Proteolytic enzyme activity in the quaking mutant mouse central nervous system (CNS) was investigated. Results showed similar enzyme levels in mutant and normal mice, suggesting proteolysis is not the cause of hypomyelination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mid-upper arm circumference predicts treatment outcomes in paediatric TB: a cohort study.

IJTLD open·2026

Same author

Experiences and perceptions of dietitians for obesity management: a general practice qualitative study.

Journal of human nutrition and dietetics : the official journal of the British Dietetic Association·2021

Same author

Aortocaval fistula, a potentially favourable complication of abdominal aortic aneurysm rupture in endovascular repair.

Annals of the Royal College of Surgeons of England·2020

Same author

Development of a group structured education programme to support safe exercise in people with Type 1 diabetes: the EXTOD education programme.

Diabetic medicine : a journal of the British Diabetic Association·2019

Same author

Preliminary report: Surgical outcomes following genitoplasty in children with moderate to severe genital atypia.

Journal of pediatric urology·2018

Same author

Role of Calpain in Spinal Cord Injury: Effects of Calpain and Free Radical Inhibitors<sup>a</sup>.

Annals of the New York Academy of Sciences·2017

Area of Science:

Neuroscience
Biochemistry
Genetics

Background:

The quaking mouse is a model for hypomyelination.
Increased proteolytic activity has been hypothesized to contribute to hypomyelination in this mutant.

Purpose of the Study:

To investigate the activity of six specific proteolytic enzymes in the central nervous system (CNS) of quaking mutant mice.
To determine if altered proteolytic activity is associated with the hypomyelination characteristic of the quaking mutation.

Main Methods:

Assayed activity of cathepsin B-like enzyme, cathepsin D, neutral proteinase, calcium-activated neutral proteinase, prolyl endopeptidase, and diaminopeptidase II.
Enzyme activity was measured in whole homogenates of brain and spinal cord from quaking mutant and normal mice.

Main Results:

All six assayed proteolytic enzymes exhibited similar activity levels in the CNS of quaking mutant mice compared to normal mice.
No significant differences in the activity of these enzymes were detected between the mutant and control groups.

Conclusions:

The findings do not support a direct relationship between increased proteolytic activity and the genetic defect causing hypomyelination in quaking mice.
Other etiological factors should be explored to understand the pathogenesis of hypomyelination in this neurological mutant model.

Related Experiment Videos