Aspartate-like immunoreactivity in mitral cells of rat olfactory bulb

Antisera against aspartate (Asp) were prepared by immunizing rabbits with Asp conjugated to bovine serum albumin by glutaraldehyde, after which the antisera were purified using an Asp-immobilized epoxy-activated affinity column. The purified Asp antiserum showed no cross-reactivity, except for a 3% cross-reactivity against D-Asp. Asp-like immunoreactivity in mitral cells of the rat olfactory bulb was demonstrated, using this purified Asp antiserum.