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Related Concept Videos

Veins01:17

Veins

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Veins are an integral part of our circulatory system, serving as the blood vessels that transport blood from all body regions to the heart. They are a network of hollow tubes that carry blood low in oxygen from the body's cells back to the heart for reoxygenation. Veins are crucial for maintaining the body's overall fluid balance and the continuous circulation of blood.
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The azygos system is a crucial part of the body's circulatory system and drains most of the thorax. It comprises the azygos, hemiazygos, and accessory hemiazygos veins.
The azygos vein, positioned just right of the midline and anterior to the vertebral column, begins at the junction of the right ascending lumbar and subcostal veins, terminating in the superior vena cava. This vein drains blood from the right side of the thoracic wall, thoracic viscera, and posterior abdominal wall.
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Veins of Lower Limbs01:15

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The human body consists of an intricate network of veins responsible for the crucial task of blood drainage from the lower limbs. These veins can be categorized into two main types: deep veins and superficial veins.
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Response Surface Methodology

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Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
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Veins as Blood Reservoirs01:10

Veins as Blood Reservoirs

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Veins, while chiefly responsible for circulating blood back to the heart, also function as storage vessels for blood. They house approximately 64 percent of the body's total blood volume, a feat made possible by their high capacitance—the inherent ability to expand and accommodate large volumes of blood, even under low pressure. The large diameter and thin walls of veins augment their distensibility, significantly more so than arteries, due to their classification as capacitance...
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Overview of Systemic Veins01:11

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Systemic veins are crucial blood vessels that return deoxygenated blood from various body tissues back to the heart. There are three systemic veins that return deoxygenated blood to the heart, they are as follows.
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Updated: Feb 6, 2026

Decellularization and Recellularization Methodology for Human Saphenous Veins
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Decellularization and Recellularization Methodology for Human Saphenous Veins

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Decellularization and Recellularization Methodology for Human Saphenous Veins.

Vijay Kumar Kuna1, Bo Xu2, Suchitra Sumitran-Holgersson2

  • 1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg; vijay.kuna.kumar@gu.se.

Journal of Visualized Experiments : Jove
|August 14, 2018
PubMed
Summary
This summary is machine-generated.

This study details a novel tissue engineering method for creating personalized vascular grafts from human saphenous veins. The decellularization and recellularization process shows promise for improved vascular surgery outcomes.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Vascular Surgery

Background:

  • Current vascular grafts (allogeneic, synthetic) have limitations including immunosuppression and poor patency.
  • Tissue engineering offers a solution for personalized grafts using decellularized extracellular matrix and recipient cells.

Purpose of the Study:

  • To detail a method for decellularizing human saphenous veins.
  • To demonstrate recellularization of decellularized veins using peripheral blood and growth factors.

Main Methods:

  • Decellularization using Triton X-100, tri-n-butyl-phosphate (TnBP), and deoxyribonuclease (DNase) in cycles.
  • Sterilization with peracetic acid and preconditioning in endothelial medium.
  • Recellularization via perfusion with peripheral blood, growth factors (VEGF, bFGF), and acetyl salicylic acid in a bioreactor.

Main Results:

  • Successful decellularization of the saphenous vein, confirmed by visual appearance (white) and H&E staining (absence of nuclei).
  • Recellularization resulted in cells present on the luminal surface of the vein, indicated by H&E staining.
  • The method provides a detailed protocol for engineered vascular conduit creation.

Conclusions:

  • The described decellularization and recellularization method offers a potential strategy for generating patient-specific vascular grafts.
  • This approach may overcome limitations associated with current allogeneic and synthetic grafts.
  • Further research can explore the long-term patency and integration of these engineered grafts in vivo.