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Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Preparation of Pyrimidine Alkenyl Acyclic Nucleoside Phosphonoamidates
Elisa Pileggi1, Michaela Serpi1, Fabrizio Pertusati1
1School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, United Kingdom.
Abstract:
This synthetic protocol describes two strategies for the preparation of pyrimidine alkenyl acyclic nucleoside phosphonoamidates (ANPs), including linear and trisubstituted alkenyl derivatives. For the first procedure, a bis-trimethylsilyl ester of the parent alkenyl ANPs is the key intermediate that reacts with the desired amino acid ester and aryl alcohol. For the second procedure, an allyl phosphonoamidate bearing the ProTide promoieties is the key synthon employed as olefin partner for a cross-metathesis reaction with an alkylated nucleobase. © 2018 by John Wiley & Sons, Inc.
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