Related Experiment Video

Updated: Feb 6, 2026

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

miR-486 inhibited osteosarcoma cells invasion and epithelial-mesenchymal transition by targeting PIM1

Yuhan Liu1,1, Juan Zhang2,1, Cuihong Xing3

  • 1The First Department of Orthopedics, Qingdao West Coast New Area Central Hospital, Qingdao, Shandong, China.

Cancer Biomarkers : Section a of Disease Markers
|August 15, 2018
PubMed
Abstract

Insights

MicroRNA-486 (miR-486) acts as a tumor suppressor in osteosarcoma by inhibiting cell invasion and epithelial-mesenchymal transition (EMT). Its downregulation, alongside PIM1 overexpression, predicts a poor prognosis for osteosarcoma patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Osteosarcoma is a common bone cancer with a high recurrence rate.
  • MicroRNA-486 (miR-486) functions as a tumor suppressor in various cancers.
  • The role of miR-486 in osteosarcoma cell invasion and epithelial-mesenchymal transition (EMT) requires further investigation.

Purpose of the Study:

  • To investigate the role of miR-486 in osteosarcoma.
  • To explore the mechanism by which miR-486 affects osteosarcoma cell invasion and EMT.
  • To determine the relationship between miR-486 and PIM1 in osteosarcoma.

Main Methods:

  • Analysis of miR-486 and PIM1 expression levels in osteosarcoma tissues.
  • Correlation analysis between miR-486 and PIM1 expression.
  • In vitro experiments to assess the effects of miR-486 upregulation or PIM1 downregulation on osteosarcoma cell invasion and EMT.
  • Luciferase reporter assays to confirm the binding of miR-486 to the PIM1 mRNA 3'-UTR.
  • Prognostic analysis of miR-486 and PIM1 expression in osteosarcoma patients.

Main Results:

  • miR-486 was found to be downregulated in osteosarcoma, while PIM1 was overexpressed, exhibiting a negative correlation.
  • Upregulation of miR-486 or downregulation of PIM1 significantly inhibited osteosarcoma cell invasion and EMT.
  • miR-486 directly targets PIM1 mRNA's 3'-UTR, mediating PIM1 expression.
  • PIM1 partially mediated the effects of miR-486 on osteosarcoma invasion.
  • Low miR-486 expression or high PIM1 expression correlated with a poor prognosis in osteosarcoma patients.

Conclusions:

  • miR-486 suppresses osteosarcoma cell invasion and EMT by targeting PIM1.
  • The miR-486/PIM1 axis offers a novel perspective on osteosarcoma pathogenesis.
  • Aberrant miR-486 expression or PIM1 overexpression serves as a potential biomarker for predicting poor prognosis in osteosarcoma.
Keywords:
EMTPIM1invasionmiR-486osteosarcoma

More Related Videos

Induction and Analysis of Epithelial to Mesenchymal Transition10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

36.6K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.4K

Related Concept Videos

Phase Transitions02:31

Phase Transitions

Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.2K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.6K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.2K
Properties of Transition Metals02:58

Properties of Transition Metals

Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
29.9K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.8K
Phase Transitions: Vaporization and Condensation02:39

Phase Transitions: Vaporization and Condensation

The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules...
21.5K