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Lymphocyte Deficiency Induced by Sublethal Irradiation in Xenopus
Louise A Rollins-Smith1,2, Jacques Robert3
1Departments of Pathology, Microbiology, and Immunology and of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232; louise.rollins-smith@vanderbilt.edu.
Cold Spring Harbor Protocols
|August 15, 2018
Summary
This study details a method using sublethal irradiation to reduce lymphocyte numbers in Xenopus laevis, aiding research into amphibian immune responses against pathogens like Batrachochytrium dendrobatidis.
Area of Science:
- Amphibian immunology
- Immunosuppression techniques
Background:
- Understanding amphibian immune responses is crucial for disease research.
- Distinguishing between innate and adaptive immunity is key for pathogen resistance.
Purpose of the Study:
- To describe a protocol for sublethal irradiation in Xenopus laevis.
- To reduce lymphocyte populations for experimental manipulation.
Main Methods:
- Adult or larval Xenopus laevis were exposed to 9 Gy of irradiation.
- Irradiation was delivered using a cesium source or gamma irradiator.
- This method targets spleen lymphocyte populations.
Main Results:
- Sublethal irradiation effectively reduces lymphocyte numbers.
- The protocol provides a tool for studying lymphocyte-mediated immunity.
Conclusions:
- Irradiation is a viable method for modulating lymphocyte levels in Xenopus laevis.
- This technique facilitates research into amphibian disease resistance mechanisms.
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