Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complementary DNA01:44

Complementary DNA

31.7K
Overview
31.7K
What is a Nervous System?01:25

What is a Nervous System?

105.1K
Overview
105.1K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.8K
Disorders of the Nervous Tissue01:28

Disorders of the Nervous Tissue

2.7K
Nervous tissue is a vital component of the human body's communication system, enabling us to perceive and respond to stimuli. However, like all other tissues, it is vulnerable to disorders and diseases that can significantly impact our neurological functioning.
Homeostatic Imbalances:
Alzheimer's disease manifests as a gradual decline in memory and cognitive abilities, attributed to the buildup of amyloid plaques and neurofibrillary tangles in the brain.
Parkinson's disease arises from the...
2.7K
Nervous Tissue: Myelin01:25

Nervous Tissue: Myelin

5.9K
The myelin sheath is a multilayered lipid and protein covering that insulates the axon of a neuron, enhancing the speed of nerve impulse conduction. Axons without this sheath are referred to as unmyelinated. Two types of neuroglia, Schwann cells in the peripheral nervous system (PNS) and oligodendrocytes in the central nervous system (CNS) are responsible for producing myelin sheaths.
Schwann cells begin to form myelin sheaths around axons during fetal development. They wrap around a small...
5.9K
The Central Dogma01:25

The Central Dogma

139.9K
Overview
139.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment with KCL-286, a first-in-class retinoic acid receptor-β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease.

FEBS open bio·2026
Same author

Beech latewood density as a proxy for temperature reconstruction.

Science advances·2026
Same author

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same author

The embryonic origins of site-specific arthritis.

Nature immunology·2026
Same author

The Platelet/Megakaryocyte Axis is Necessary for Allergic Sensitisation and Inflammatory Responses to House Dust Mite in the Lung.

Lung·2026
Same author

Ex vivo human placental imaging: Navigating modalities, scales, and analysis approaches to obtain fit-for-purpose data.

Placenta·2026

Related Experiment Video

Updated: Feb 6, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K

Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system

Merrick C Strotton1, Andrew J Bodey2, Kazimir Wanelik2

  • 1King's College London, Wolfson Centre for Age Related Diseases, Institute of Psychiatry, Psychology & Neuroscience, Guy's Campus, London Bridge, London, SE1 1UL, UK.

Scientific Reports
|August 15, 2018
PubMed
Summary

Synchrotron radiation microtomography (SRmicroCT) offers fast, high-resolution 3D imaging of soft tissues. This optimized method visualizes rat spinal cord structures, preserving tissue for histology and informing future nervous system research.

More Related Videos

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography
08:11

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

Published on: August 26, 2015

9.3K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Related Experiment Videos

Last Updated: Feb 6, 2026

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K
Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography
08:11

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

Published on: August 26, 2015

9.3K
Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

10.5K

Area of Science:

  • Biomedical Imaging
  • Neuroscience
  • Materials Science

Background:

  • Synchrotron radiation microtomography (SRmicroCT) is a powerful non-destructive 3D imaging technique for soft tissues.
  • Optimizing sample preparation, scanning, and signal processing enhances SRmicroCT efficiency, quality, and data utility.

Purpose of the Study:

  • To develop and optimize a novel SRmicroCT methodology for rapid, high-resolution imaging of the rat spinal cord.
  • To demonstrate the preservation of spinal cord tissue for subsequent histological analysis.

Main Methods:

  • Evaluated various sample preparations (embedding media, stains), imaging parameters (projection number, propagation distance), and reconstruction techniques (artefact correction, phase retrieval).
  • Optimized a method combining reversible iodine staining, wax embedding, and inline phase contrast for SRmicroCT.
  • Utilized a 'single-scan iterative downsampling' approach for efficient data acquisition.

Main Results:

  • Achieved fast (~12 minutes) high-resolution imaging of a 3.5 mm rat spinal cord segment, resolving capillaries down to 3.2-4.8 micrometers.
  • Successfully segmented white-grey matter macro- and micro-features, including motoneurons and vasculature, using machine learning.
  • Demonstrated that SRmicroCT-imaged tissue remains suitable for subsequent histology.

Conclusions:

  • The optimized SRmicroCT methodology provides efficient, high-resolution 3D imaging of nervous system tissues.
  • This technique is valuable for studying spinal cord pathologies and other neural tissues, complementing traditional histology.
  • The study provides guidance for designing future soft tissue SRmicroCT experiments.