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ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions
Przemyslaw Wanat1, Renata Kasprzyk, Michal Kopcial
1Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland. jkowalska@fuw.edu.pl.
Abstract:
We describe a new type of nucleotide-derived fluorescent probe designed for monitoring pyrophosphatase activity based on excimer-to-monomer transitions, called ExciTide. The nucleotides were designed with two self-interacting dye moieties and synthesised using copper-catalysed azide-alkyne cycloaddition click chemistry. We applied these probes for enzyme activity monitoring and inhibitor evaluation. Some of the probes permeated into living cells, yielding interesting prospects for future applications.
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