Related Experiment Video

Updated: Feb 6, 2026

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
09:58

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

8.1K

miR-129 targets CDK1 and iASPP to modulate Burkitt lymphoma cell proliferation in a TAp63-dependent manner

Hui Zou1, Runying Zou1, Keke Chen1

  • 1Department of Hematology and Oncology of Children's Medical Center, The First Affiliated Hospital of Hunan Normal University/Hunan Provincial People's Hospital, Changsha, Hunan, China.

Journal of Cellular Biochemistry
|August 15, 2018
PubMed

Insights

MicroRNA-129 suppresses Burkitt lymphoma by targeting CDK1 and iASPP, restoring TAp63-mediated cell proliferation inhibition. This finding offers a potential therapeutic strategy for this aggressive cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Genetics

Background:

  • Burkitt lymphoma is an aggressive cancer with poor outcomes in adults.
  • p53-induced apoptosis is crucial for tumor suppression, but p53 is often mutated in Burkitt lymphoma.
  • The p53 family member, p63, is a potential therapeutic target in cancer.

Purpose of the Study:

  • To investigate the role of iASPP in TAp63-dependent cell proliferation inhibition in Burkitt lymphoma.
  • To elucidate the regulatory mechanism of iASPP and TAp63 by microRNA-129.

Main Methods:

  • Verification of iASPP's oncogenic effect dependency on TAp63.
  • Analysis of the interaction between CDK1 and iASPP.
  • In silico prediction and experimental validation of miR-129 binding to iASPP and CDK1.
  • Assessment of miR-129 inhibitor and CDK1 knockdown effects on protein levels and localization.

Main Results:

  • iASPP promotes Burkitt lymphoma proliferation in a TAp63-dependent manner.
  • miR-129 acts as a tumor suppressor by inhibiting CDK1 and iASPP expression and cancer cell proliferation.
  • miR-129 targets CDK1, reducing iASPP phosphorylation and nuclear localization, thereby diminishing its inhibitory effect on p53 and TAp63.

Conclusions:

  • miR-129 directly inhibits CDK1 and iASPP expression in Burkitt lymphoma.
  • Targeting CDK1 by miR-129 restores TAp63-induced proliferation inhibition, highlighting miR-129 as a potential therapeutic agent.
Keywords:
Burkitt lymphomaTAp63cyclin-dependent kinase 1 (CDK1)iASPPp53proliferation

More Related Videos

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma08:31

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma

Published on: September 12, 2025

836
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

14.8K

Related Concept Videos

Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
Frequency-dependent Selection01:21

Frequency-dependent Selection

When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
24.1K
Contact-dependent Signaling01:19

Contact-dependent Signaling

Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.6K
Drug Dependence01:17

Drug Dependence

Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
Target Cell Response to Hormones01:22

Target Cell Response to Hormones

Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
5.8K