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Microarray analysis of miRNAs during hindgut development in rat embryos with ethylenethiourea‑induced anorectal

Cai-Yun Long1, Xiao-Bing Tang1, Wei-Lin Wang1

  • 1Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China.

International Journal of Molecular Medicine
|August 15, 2018
PubMed

Insights

This study investigated microRNAs (miRNAs) in rats with anorectal malformations (ARMs). Key miRNAs, including miR-125b-2-3p, miR-92a-2-5p, and miR-99a-5p, were found to be differentially expressed, suggesting their role in ARM pathogenesis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Anorectal malformations (ARMs) are common congenital digestive tract defects with unclear pathogenesis.
  • MicroRNAs (miRNAs) play crucial roles in gastrointestinal development and are implicated in ARM development.

Purpose of the Study:

  • To profile miRNAs and investigate their potential functions in ethylenethiourea (ETU)-induced ARMs in a rat model.
  • To identify specific miRNAs involved in the key developmental stages of anorectal formation.

Main Methods:

  • Pregnant Wistar rats were exposed to ETU on gestational day 10 to induce ARMs.
  • Hindgut tissues from rat embryos were analyzed using microarray and RT-qPCR at gestational days 14, 15, and 16.
  • Bioinformatics analysis was performed on differentially expressed miRNAs.

Main Results:

  • 38, 49, and 42 miRNAs were differentially expressed on gestational days 14, 15, and 16, respectively, in the ARM group compared to controls.
  • Five differentially expressed miRNAs were identified to be involved in ARM-associated signaling pathways.
  • miR-125b-2-3p, miR-92a-2-5p, and miR-99a-5p showed significant differential expression in rats with ARMs.

Conclusions:

  • The study identified specific miRNAs, including miR-125b-2-3p, miR-92a-2-5p, and miR-99a-5p, that are differentially expressed during critical periods of anorectal formation in rats.
  • These findings suggest that these miRNAs may play a significant role in the pathogenesis of anorectal malformations.

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