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Related Concept Videos

Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
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Muscle Stem Cell Immunostaining.

Shuaiyu Wang1, Bao Zhang1, Gregory C Addicks2

  • 1Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education and The Department of Histology and Embryology of Zhongshan School of Medicine, Sun-Yat Sen University, Guangzhou, China.

Current Protocols in Mouse Biology
|August 15, 2018
PubMed
Summary

This study details a method for characterizing muscle stem cells (MuSCs) in mice. Understanding MuSC behavior is crucial for regenerative medicine, particularly for Duchenne Muscular Dystrophy and aging.

Keywords:
immunostainingmuscle stem cell (MuSC)skeletal muscle

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Area of Science:

  • Muscle biology and regenerative medicine

Background:

  • Muscle stem cells (MuSCs) are vital for muscle repair and homeostasis.
  • MuSC function is altered in conditions like Duchenne Muscular Dystrophy (DMD) and during aging.
  • Characterizing MuSC states (activation, senescence, apoptosis) is key for therapeutic development.

Purpose of the Study:

  • To present a detailed in situ method for characterizing mouse muscle stem cells.
  • To enable analysis of MuSC properties relevant to muscle regeneration and disease.

Main Methods:

  • Utilizes in situ immunostaining techniques on mouse skeletal muscle.
  • Adaptable protocol for analyzing various skeletal muscle properties.

Main Results:

  • Provides a reproducible method for MuSC characterization.
  • Enables detailed assessment of MuSC proliferation, activation, senescence, and apoptosis.

Conclusions:

  • The described immunostaining method is effective for in situ MuSC characterization in mice.
  • This technique can be applied to study muscle regeneration, aging, and diseases like DMD.