Related Experiment Video
Updated: Feb 6, 2026
10:23
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
9.4K
Identification of Missing Proteins in Normal Human Cerebrospinal Fluid
Charlotte Macron1, Lydie Lane2,3, Antonio Núñez Galindo1
1Proteomics , Nestlé Institute of Health Sciences , 1015 Lausanne , Switzerland.
Journal of Proteome Research
|August 15, 2018
Summary
Researchers analyzed the human cerebrospinal fluid (CSF) proteome, identifying 2,281 proteins. This study aims to validate the existence of seven proteins currently missing from proteomic databases.
Area of Science:
- Proteomics
- Biochemistry
- Human Physiology
Background:
- The cerebrospinal fluid (CSF) proteome contains vital biomarkers for neurological health.
- Comprehensive proteomic profiling of CSF is essential for understanding brain function and disease.
- The Chromosome-centric Human Proteome Project (C-HPP) aims to identify all human proteins.
Purpose of the Study:
- To characterize the human cerebrospinal fluid (CSF) proteome.
- To identify proteins, including potential 'missing proteins', within the CSF.
- To contribute data to the Chromosome-centric Human Proteome Project (C-HPP).
Main Methods:
- Immunodepletion of abundant proteins from normal human CSF.
- Off-gel electrophoresis for protein fractionation.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a linear ion trap-Orbitrap Elite analyzer.
Main Results:
- Identification of 12,344 peptides.
- Mapping of identified peptides to 2,281 distinct proteins.
- Proposal for the validation of seven 'missing proteins' within the human proteome.
Conclusions:
- This study provides a significant expansion of the known human CSF proteome.
- The identified proteins and data contribute to the goals of the C-HPP.
- The dataset is publicly available for further research and validation of novel protein findings.
Related Concept Videos
01:21Cerebrospinal Fluid
6.0K
Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
6.0K
01:11Normal Distribution
17.3K
The normal, a continuous distribution, is the most important of all the distributions. Its graph is a bell-shaped symmetrical curve, which is observed in almost all disciplines. Some of these include psychology, business, economics, the sciences, nursing, and, of course, mathematics. Some instructors may use the normal distribution to help determine students’ grades. Most IQ scores are normally distributed. Often real-estate prices fit a normal distribution. The normal distribution is...
17.3K
01:19Normal Stress
1.4K
Normal stress is a type of stress that occurs when forces act perpendicular, or normal, to a material's cross-sectional area. This stress often arises in structures when subjected to axial loading, which is the application of force along the axis of an object. A practical example of this can be found in bridge truss members.
When a rod is under axial loading, the internal forces and corresponding stress are normal to the plane of the section, so it is termed normal stress. It's important to...
When a rod is under axial loading, the internal forces and corresponding stress are normal to the plane of the section, so it is termed normal stress. It's important to...
1.4K
01:34The Fluid Mosaic Model
178.7K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
178.7K
01:22Applications of Normal Distribution
9.5K
The normal distribution is a useful statistical tool. One of its practical applications is determining the door height after considering the normal distribution of heights of persons, such that many can pass through it easily without striking their heads. The normal distribution can also determine the probability of a person having a height less than a specific height.
The heights of 15 to 18-year-old males from Chile from 1984 to 1985 followed a normal distribution. The mean height is 172.36...
The heights of 15 to 18-year-old males from Chile from 1984 to 1985 followed a normal distribution. The mean height is 172.36...
9.5K
01:29Introduction to Normal Distributions
80
Standardized test scores often follow a symmetric distribution that can be modeled with the normal distribution, a fundamental concept in statistics. This distribution is particularly useful for interpreting test performance fairly across populations, as it provides a mathematical framework for understanding variability and central tendency in large datasets.From Histogram to Frequency DistributionRaw test data are often displayed using histograms, where the height of each bar represents the...
80