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Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
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Ultrafast optical imaging at 2.0 μm through second-harmonic-generation-based time-stretch at 1.0 μm
Optics Letters
|August 15, 2018
Summary
This study introduces an ultrafast optical imaging system for 2.0-μm wavelength imaging. By combining second-harmonic generation (SHG) with time-stretch detection, it achieves high sensitivity and a fast line scan rate.
Area of Science:
- Optical Imaging
- Spectroscopy
- Biomedical Optics
Background:
- Ultrafast time-stretch imaging performance is limited at long wavelengths (>1.5 μm) by optical fiber losses.
- Low detection sensitivity hinders applications in spectral regions with unique optical absorption contrast.
Purpose of the Study:
- To develop an ultrafast optical imaging system with enhanced detection sensitivity at 2.0 μm.
- To enable time-stretch imaging at longer wavelengths by overcoming fiber loss limitations.
Main Methods:
- Utilized second-harmonic generation (SHG) to convert 2.0-μm signals to 1.0 μm.
- Employed highly sensitive time-stretch detection at 1.0 μm.
- Achieved spectral encoding of the sample at 2.0 μm, followed by temporal mapping via a dispersive fiber.
Main Results:
- Demonstrated an ultrafast optical imaging system with a line scan rate of approximately 19 MHz at 2.0 μm.
- Achieved a superior dispersion-to-loss ratio of ~53 ps/nm/dB at 1.0 μm, ~50 times greater than standard fibers at 2.0 μm.
- Successfully translated time-stretch technology to longer wavelengths while maintaining high detection sensitivity.
Conclusions:
- The developed system overcomes sensitivity limitations of long-wavelength time-stretch imaging.
- This advancement allows leveraging unique optical absorption contrasts at longer wavelengths.
- The approach benefits from high detection sensitivity typically found at shorter wavelengths.
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