Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

7.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.6K
Molecular Comparison of Gases, Liquids, and Solids02:26

Molecular Comparison of Gases, Liquids, and Solids

55.3K
Particles in a solid are tightly packed together (fixed shape) and often arranged in a regular pattern; in a liquid, they are close together with no regular arrangement (no fixed shape); in a gas, they are far apart with no regular arrangement (no fixed shape). Particles in a solid vibrate about fixed positions (cannot flow) and do not generally move in relation to one another; in a liquid, they move past each other (can flow) but remain in essentially constant contact; in a gas, they move...
55.3K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.5K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.5K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

1.0K
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
1.0K
Separable Differential Equations01:20

Separable Differential Equations

105
A separable differential equation is a type of first-order differential equation where the derivative dy/dx can be expressed as a product of two functions: one that depends only on x and another that depends only on y. This allows for the rearrangement of the equation so that all terms involving y are on one side, and all terms involving x are on the other. This process, known as the separation of variables, simplifies the process of solving the equation by enabling the integration of both...
105
Types of Building Separation Joints01:23

Types of Building Separation Joints

622
Building separation joints divide large or complex building structures into smaller, discrete units that can move independently. These joints are categorized into three types: volume-change joints, settlement joints, and seismic separation joints.
Volume-change joints address the effects of expansion and contraction due to temperature and moisture variations. They are strategically placed at discontinuities in a building's mass where cracking is most likely and are spaced about 150 to 200...
622

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicenter validation of plasma p-tau217/ amyloid beta 1-42 ratio in symptomatic Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Uncomplicated type B aortic dissection: Are US-based societal guidelines delaying science?

The Annals of thoracic surgery·2026
Same author

Plasma pTau 217/β-amyloid 1-42 ratio for enhanced accuracy and reduced uncertainty in detecting amyloid pathology.

Brain : a journal of neurology·2026
Same author

The utility of the novel biomarker HE4 for monitoring epithelial ovarian cancer during PARP inhibitor treatment.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2025
Same author

Identification of circulating tumor cells captured by the FDA-cleared Parsortix<sup>®</sup> PC1 system from the peripheral blood of metastatic breast cancer patients using immunofluorescence and cytopathological evaluations.

Journal of experimental & clinical cancer research : CR·2024
Same author

Lumbar Paraspinal Compartment Syndrome in an Active-Duty Army Special Operations Aviation Soldier.

Journal of special operations medicine : a peer reviewed journal for SOF medical professionals·2024

Related Experiment Video

Updated: Feb 6, 2026

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

4.6K

The Parsortix™ Cell Separation System-A versatile liquid biopsy platform.

M Craig Miller1, Peggy S Robinson2, Christopher Wagner3

  • 1ANGLE North America, Inc., Clinical Development, King of Prussia, Pennsylvania.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|August 15, 2018
PubMed
Summary

The Parsortix™ system offers a new microfluidic technology for capturing circulating tumor cells (CTCs) based on size and deformability. This method provides highly enriched, viable CTCs for downstream analysis, addressing challenges in liquid biopsy technology selection.

Keywords:
circulating tumor cellliquid biopsymicrofluidicsrare cell isolation

More Related Videos

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

11.0K
Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
14:54

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Published on: September 11, 2023

3.2K

Related Experiment Videos

Last Updated: Feb 6, 2026

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

4.6K
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

11.0K
Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
14:54

Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

Published on: September 11, 2023

3.2K

Area of Science:

  • Oncology
  • Biotechnology
  • Cell Biology

Background:

  • Circulating tumor cells (CTCs) are shed from solid tumors into the bloodstream and contribute to metastasis.
  • The CellSearch® system was the first FDA-cleared platform for CTC detection, but advancements are needed.
  • The field of liquid biopsy is rapidly expanding with diverse technologies for CTC analysis.

Purpose of the Study:

  • To introduce and evaluate the Parsortix™ Cell Separation System, a novel microfluidic technology for CTC isolation.
  • To assess the Parsortix™ system's ability to capture, enrich, and maintain the viability of CTCs.
  • To highlight the Parsortix™ system's utility for various downstream analyses, including single-cell interrogation.

Main Methods:

  • The Parsortix™ system utilizes microfluidics to capture rare cells based on physical properties like size and deformability.
  • The technology focuses on reproducible high capture efficiency for CTCs.
  • Isolated CTCs are viable and suitable for extensive downstream molecular and cellular analyses.

Main Results:

  • The Parsortix™ system demonstrates reproducible high capture efficiency for CTCs.
  • The system yields highly enriched and viable CTCs.
  • The captured CTCs are amenable to diverse downstream applications, including single-cell analysis.

Conclusions:

  • The Parsortix™ system represents a promising advancement in CTC isolation technology.
  • This microfluidic platform offers a reliable and efficient method for obtaining viable CTCs for liquid biopsy.
  • The Parsortix™ system provides a valuable tool for researchers investigating CTCs and their role in cancer progression and treatment.