Related Experiment Video
Updated: Feb 6, 2026
05:29
Author Spotlight: Investigating Wolbachia-Induced Thelytokous Parthenogenesis and Genetic Toolkit Development Through RNA Interference
Published on: November 21, 2023
1.9K
Transcriptome characterization and gene expression analysis related to chemoreception in Trichogramma chilonis, an
Jian-Bai Liu1, Han Wu1, Jie-Qun Yi1
1State Key Laboratory for Biocontrol, Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, China.
Gene
|August 15, 2018
Summary
This study identifies 85 chemosensory genes in Trichogramma chilonis, revealing molecular insights into insect olfaction. Key odorant receptor and binding protein genes show male-biased expression, crucial for biocontrol applications.
Area of Science:
- Entomology
- Molecular Biology
- Genomics
Background:
- Chemoreception is vital for insect survival, influencing behaviors like mating and host-searching.
- Trichogramma chilonis is an important biocontrol agent, but its olfactory mechanisms are poorly understood.
Purpose of the Study:
- To explore sex-specific differences in chemoreception by analyzing the transcriptome of T. chilonis.
- To identify candidate chemosensory genes involved in olfaction-evoked behaviors.
Main Methods:
- Transcriptome profiling using Illumina high-throughput sequencing.
- Identification and quantification of chemosensory genes, including odorant receptors (ORs) and odorant binding proteins (OBPs).
- Validation of gene expression using quantitative real-time PCR (qRT-PCR).
Main Results:
- Identified 85 chemosensory genes: 45 ORs, 22 OBPs, 14 ionotropic receptors (IRs), 2 sensory neuron membrane proteins (SNMPs), and 2 chemosensory proteins (CSPs).
- Most olfactory genes exhibited similar expression in males and females.
- Several OR and OBP genes displayed male-biased expression, suggesting roles in sex-specific behaviors.
Conclusions:
- This study provides the first molecular identification of chemosensory genes in T. chilonis.
- The findings lay a foundation for future research into the T. chilonis chemosensory system.
- Understanding these genes can enhance the use of T. chilonis as a biological control agent.
Related Concept Videos
01:42What is Gene Expression?
196.9K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
01:36What is Gene Expression?
11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
02:35Chromatin Position Affects Gene Expression
24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
02:51mRNA Stability and Gene Expression
6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.7K