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Updated: Feb 6, 2026

Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
Published on: August 1, 2013
Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based
Cuicui Li1, Masafumi Takeo1, Masayoshi Matsuda1
1Graduate School of Systems Life Sciences , Kyushu University , 744 Motooka, Nishi-ku , Fukuoka 819-0395 , Japan . Email: ykatatcm@mail.cstm.kyushu-u.ac.jp ; Tel: +81 092 802 2850.
Abstract:
The introduction of proteins into dendritic cells (DCs) ex vivo is a critical step for the DC-based immunotherapy of cancer. Here, we developed a biotin-modified polymer with multiple hydrophobic membrane anchors for cells that functions as a synthetic receptor for an antigen protein, ovalbumin (OVA), to introduce it efficiently into DCs compared with the conventional pulsing method. Our method showed significant advantages, including the rapid incorporation of OVA and the activation of antigen-specific T cells in a MHC-restricted manner. When mice were immunized by DCs treated with our method, tumor growth was completely suppressed, indicating that our method can be used to prepare adjuvant DCs.
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