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Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS
Published on: January 22, 2017
Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass
Michaela Tvrdonova1,2, Marcela Vlcnovska3, Lucie Pompeiano Vanickova3,4
1Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00, Brno, Czech Republic.
Abstract:
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
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