Related Experiment Video
Updated: Feb 6, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
Published on: September 2, 2025
Modular Assembly of Synthetic Secondary Chromosomes
Celine Zumkeller1, Daniel Schindler1,2, Torsten Waldminghaus3
1Chromosome Biology Group, LOEWE Center for Synthetic Microbiology-SYNMIKRO, Philipps-Universität Marburg, Marburg, Germany.
Abstract:
The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular Modular Cloning system (MoClo).
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