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Updated: Feb 6, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Lipase and Phospholipase Activity Methods for Marine Organisms
H Nolasco-Soria1, F Moyano-López2, F Vega-Villasante3
1Centro de Investigaciones Biológicas del Noroeste, S.C., La Paz, Baja California Sur, Mexico. hnolasco04@cibnor.mx.
Abstract:
Lipases are very important enzymes having a role in fat digestion and lipid metabolism in marine animals, plants, and microorganisms. The methods for measuring lipase and phospholipase activity have been applied in several studies; however, considering that lipases are water-soluble molecules and their substrates are generally water-insoluble molecules, several steps are required for measuring their digestion products. After a general review of the main type of methods used in marine lipase studies, and experimental procedures, a proposal of new or improved methods is described in order to facilitate the lipase activity measurements in marine organisms.
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